更新時(shí)間:2024-06-11
我司LTEP-sm(人小細(xì)胞)的細(xì)胞培養(yǎng)基其組成成分主要有:水、氨基酸、維生素、碳水化合物、無機(jī)離子及其他一些入核酸降解物、激素等。保證運(yùn)輸中的正常生長(zhǎng),關(guān)于其價(jià)格、報(bào)價(jià)、貨期,已打造成抗體在華東地區(qū)Z大交易平臺(tái)之一,歡迎您的咨詢!
免費(fèi)咨詢:021-54720761
LTEP-sm(人小細(xì)胞)對(duì)于每種細(xì)胞因子應(yīng)仔細(xì)閱讀說明書,注意每批的細(xì)節(jié)。在使用細(xì)胞因子前,瞬時(shí)離心試劑瓶,盡可能多地收回其中的細(xì)胞因子。
LTEP-sm(人小細(xì)胞)來源可靠(源于ATCC、ECACC、DSMZ、JCRB等),活力>95%,貼壁性好。我們從試劑、包被器皿、凍存原代細(xì)胞、新鮮原代細(xì)胞、原代細(xì)胞的分子學(xué)實(shí)驗(yàn)等提供全程服務(wù)。我司擁有專業(yè)的實(shí)驗(yàn)室,可無菌操作并提供相關(guān)科研項(xiàng)目解決方案以及實(shí)驗(yàn)服務(wù)。
服務(wù)流程:
預(yù)約登記→辦理相關(guān)手續(xù)→復(fù)蘇細(xì)胞→細(xì)胞質(zhì)量檢查→發(fā)送細(xì)胞(或自己來取細(xì)胞)→細(xì)胞售后技術(shù)咨詢答疑。
養(yǎng)方法如下:
1、從手術(shù)室無菌取腦髓灰質(zhì)或白質(zhì)后,仔細(xì)剝除腦膜、血管和纖維成分,置Hanks液中漂洗一、二次。
2、置于30—50倍體積的Hanks液中,此時(shí)腦組織比較柔軟,反復(fù)吹打即制成細(xì)胞懸液。
3、把懸液注入離心管室溫中直立5—10分鐘后,細(xì)胞或細(xì)胞團(tuán)快自然下沉,脂肪等雜物易漂浮,可吸除上層、反復(fù)二、三次,即可排除脂肪成分和其它碎塊并獲較多細(xì)胞成分。
4、在沉降物中加入適量培養(yǎng)液,通過紗網(wǎng)成紗布過濾,計(jì)數(shù)并調(diào)整細(xì)胞密度。
5、接入培養(yǎng)瓶或皿中,置5%CO2溫箱中培養(yǎng)。
適應(yīng)環(huán)境過程較長(zhǎng),接種后貼壁較慢。貼壁后短期內(nèi)也可能不見細(xì)胞分裂現(xiàn)象,然而一旦生長(zhǎng)后即能迅速進(jìn)入旺盛的增殖狀態(tài)。細(xì)胞傳代可以0.25%胰酶消化處理。
我司其他相關(guān)銷售產(chǎn)品:
Cs-(036)-007524 中國(guó)倉(cāng)鼠XB淋巴細(xì)胞株/細(xì)胞雜交瘤MR1[derivativeofHB-11048]細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(036)-007525 中國(guó)倉(cāng)鼠倉(cāng)鼠肺細(xì)胞株/細(xì)胞,R1610細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(036)-007526 中國(guó)倉(cāng)鼠倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞亞株,CHO-K1細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(036)-007527 中國(guó)倉(cāng)鼠二氫葉酸還原酶缺陷細(xì)胞株/細(xì)胞,CHO/dhFr-細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(036)-007528 中國(guó)倉(cāng)鼠肺細(xì)胞株/細(xì)胞,CHL/IU[CHL-11]細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(036)-007529 中國(guó)倉(cāng)鼠肺細(xì)胞株/細(xì)胞,CHL細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
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Cs-(036)-007533 中國(guó)倉(cāng)鼠肺細(xì)胞株/細(xì)胞;CHL ,規(guī)格: >5×100000cells/瓶
Cs-(036)-007534 中國(guó)倉(cāng)鼠肺細(xì)胞株/細(xì)胞;R 1610 [R1610] ,規(guī)格: >5×100000cells/瓶
Cs-(036)-007535 中國(guó)倉(cāng)鼠肺細(xì)胞株/細(xì)胞;V79 ,規(guī)格: >5×100000cells/瓶
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Cs-(036)-007546 中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞(APP基因修飾)7WD10細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
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Cs-(036)-007568 中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞(亞系克?。〤HO1beta9,12.5Clone36細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(036)-007569 中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞(亞系克?。〤HO-5beta41,2.5CL5CarryingP5bdhfr細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(036)-007570 中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞(亞系克?。〤HO-beta2(414)CL.32pTGF-beta2(414)細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(036)-007571 中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞(亞系克隆)CHO-DUKXB11carryingpDEF202-hTPO-163細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(036)-007572 中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞(亞系克隆)CHO-DUKXB11carryingpDEF202-hTPO-P1細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(036)-007573 中國(guó)倉(cāng)鼠卵巢細(xì)胞株/細(xì)胞(亞系克?。〤HO-DUKXB11-prepro-TGFβ1cDNA-TGF-β1DindingProt ,規(guī)格: >5×100000cells/瓶
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