更新時(shí)間:2024-06-11
我司HLEC(人淋巴內(nèi)皮細(xì)胞)的細(xì)胞培養(yǎng)基其組成成分主要有:水、氨基酸、維生素、碳水化合物、無機(jī)離子及其他一些入核酸降解物、激素等。保證運(yùn)輸中的正常生長,關(guān)于其價(jià)格、報(bào)價(jià)、貨期,已打造成抗體在華東地區(qū)Z大交易平臺(tái)之一,歡迎您的咨詢!
免費(fèi)咨詢:021-54720761
HLEC(人淋巴內(nèi)皮細(xì)胞)對(duì)于每種細(xì)胞因子應(yīng)仔細(xì)閱讀說明書,注意每批的細(xì)節(jié)。在使用細(xì)胞因子前,瞬時(shí)離心試劑瓶,盡可能多地收回其中的細(xì)胞因子。
HLEC(人淋巴內(nèi)皮細(xì)胞)來源可靠(源于ATCC、ECACC、DSMZ、JCRB等),活力>95%,貼壁性好。我們從試劑、包被器皿、凍存原代細(xì)胞、新鮮原代細(xì)胞、原代細(xì)胞的分子學(xué)實(shí)驗(yàn)等提供全程服務(wù)。我司擁有專業(yè)的實(shí)驗(yàn)室,可無菌操作并提供相關(guān)科研項(xiàng)目解決方案以及實(shí)驗(yàn)服務(wù)。
服務(wù)流程:
預(yù)約登記→辦理相關(guān)手續(xù)→復(fù)蘇細(xì)胞→細(xì)胞質(zhì)量檢查→發(fā)送細(xì)胞(或自己來取細(xì)胞)→細(xì)胞售后技術(shù)咨詢答疑。
養(yǎng)方法如下:
1、從手術(shù)室無菌取腦髓灰質(zhì)或白質(zhì)后,仔細(xì)剝除腦膜、血管和纖維成分,置Hanks液中漂洗一、二次。
2、置于30—50倍體積的Hanks液中,此時(shí)腦組織比較柔軟,反復(fù)吹打即制成細(xì)胞懸液。
3、把懸液注入離心管室溫中直立5—10分鐘后,細(xì)胞或細(xì)胞團(tuán)快自然下沉,脂肪等雜物易漂浮,可吸除上層、反復(fù)二、三次,即可排除脂肪成分和其它碎塊并獲較多細(xì)胞成分。
4、在沉降物中加入適量培養(yǎng)液,通過紗網(wǎng)成紗布過濾,計(jì)數(shù)并調(diào)整細(xì)胞密度。
5、接入培養(yǎng)瓶或皿中,置5%CO2溫箱中培養(yǎng)。
適應(yīng)環(huán)境過程較長,接種后貼壁較慢。貼壁后短期內(nèi)也可能不見細(xì)胞分裂現(xiàn)象,然而一旦生長后即能迅速進(jìn)入旺盛的增殖狀態(tài)。細(xì)胞傳代可以0.25%胰酶消化處理。
我司其他相關(guān)銷售產(chǎn)品:
Cs-(035)-000457 雜交瘤細(xì)胞株/細(xì)胞HBPG11細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000458 雜交瘤細(xì)胞株/細(xì)胞HBPE9細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000459 雜交瘤細(xì)胞株/細(xì)胞HBPBA5細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000460 雜交瘤細(xì)胞株/細(xì)胞HBP55細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000461 雜交瘤細(xì)胞株/細(xì)胞HBNI8細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000462 雜交瘤細(xì)胞株/細(xì)胞HBMY-904細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000463 雜交瘤細(xì)胞株/細(xì)胞HBMO-5細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000464 雜交瘤細(xì)胞株/細(xì)胞HBMO-3細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000465 雜交瘤細(xì)胞株/細(xì)胞HBMO-2細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000466 雜交瘤細(xì)胞株/細(xì)胞HBLucky1C細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000467 雜交瘤細(xì)胞株/細(xì)胞HBL4-1-31細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000468 雜交瘤細(xì)胞株/細(xì)胞HBL3-1-54細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000469 雜交瘤細(xì)胞株/細(xì)胞HBL20細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000470 雜交瘤細(xì)胞株/細(xì)胞HBKC-57細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000471 雜交瘤細(xì)胞株/細(xì)胞HBKC-4細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000472 雜交瘤細(xì)胞株/細(xì)胞HBKC-4G3細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000473 雜交瘤細(xì)胞株/細(xì)胞HBKC-48細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000474 雜交瘤細(xì)胞株/細(xì)胞HBKC-16(IgG1)細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000475 雜交瘤細(xì)胞株/細(xì)胞HBJDSHCNS5-1細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000476 雜交瘤細(xì)胞株/細(xì)胞HBJDSHCNS4-93細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000477 雜交瘤細(xì)胞株/細(xì)胞HBJDSHCNS3-1細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000478 雜交瘤細(xì)胞株/細(xì)胞HBJDSHCE125細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000479 雜交瘤細(xì)胞株/細(xì)胞HBJDSHCC39細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000480 雜交瘤細(xì)胞株/細(xì)胞HBHepama-1細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000481 雜交瘤細(xì)胞株/細(xì)胞HBHepama-18(HP-18)細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000482 雜交瘤細(xì)胞株/細(xì)胞HBHepama-1(HP-1)細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000483 雜交瘤細(xì)胞株/細(xì)胞HBG3-5細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000484 雜交瘤細(xì)胞株/細(xì)胞HBG19-4細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000485 雜交瘤細(xì)胞株/細(xì)胞HBG17-2細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000486 雜交瘤細(xì)胞株/細(xì)胞HBG10-1細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000487 雜交瘤細(xì)胞株/細(xì)胞HBCEP24G-9G4細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000488 雜交瘤細(xì)胞株/細(xì)胞HBCEP16-2B細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
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Cs-(035)-000490 雜交瘤細(xì)胞株/細(xì)胞HBBrE-1細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000491 雜交瘤細(xì)胞株/細(xì)胞HBBR96細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000492 雜交瘤細(xì)胞株/細(xì)胞HBBFR87-70細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000493 雜交瘤細(xì)胞株/細(xì)胞HBBFR87-124細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000494 雜交瘤細(xì)胞株/細(xì)胞HBBADCPPF1-75A細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
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Cs-(035)-000496 雜交瘤細(xì)胞株/細(xì)胞HBBADCPPF1-4C細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000497 雜交瘤細(xì)胞株/細(xì)胞HBBADCPPF1-35B細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000498 雜交瘤細(xì)胞株/細(xì)胞HBBADCPPF1-28A細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000499 雜交瘤細(xì)胞株/細(xì)胞HBBADCPPF1-151K細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000500 雜交瘤細(xì)胞株/細(xì)胞HB9.6細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000501 雜交瘤細(xì)胞株/細(xì)胞HB9.4細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000502 雜交瘤細(xì)胞株/細(xì)胞HB8H7A細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000503 雜交瘤細(xì)胞株/細(xì)胞HB5F12AD3細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000504 雜交瘤細(xì)胞株/細(xì)胞HB4G12-13細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000505 雜交瘤細(xì)胞株/細(xì)胞HB388F1細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(035)-000506 雜交瘤細(xì)胞株/細(xì)胞HB2G10細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
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Cs-(035)-000508 雜交瘤細(xì)胞株/細(xì)胞HB23M-D9細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
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