更新時間:2016-06-03
我司所提供的實驗KiMA:人胚腎上皮細(xì)胞系,不能用于人體實驗和臨床診斷、治療。我司細(xì)胞研究中心承諾,盡Z大努力對實驗細(xì)胞資源相關(guān)信息進(jìn)行及時更新,同時也保證純度在90%以上,咨詢購買。
免費(fèi)咨詢:021-54720761
KiMA:人胚腎上皮細(xì)胞系培養(yǎng)方法:
收到細(xì)胞后,在倒置顯微鏡下觀察整個細(xì)胞生長情況,如果細(xì)胞未長滿,用75%酒精噴灑整個瓶消毒后放到超菌臺內(nèi),嚴(yán)格無菌操作,打開細(xì)胞培養(yǎng)瓶,留10ml培養(yǎng)液繼續(xù)培養(yǎng)。
KiMA:人胚腎上皮細(xì)胞系初代培養(yǎng)原理:
將動物機(jī)體的各種組織從機(jī)體中取出,經(jīng)各種酶(常用胰蛋白酶)、螯合劑(常用EDTA)或機(jī)械方法處理,分散成單細(xì)胞,置合適的培養(yǎng)基中培養(yǎng),使細(xì)胞得以生存、生長和繁殖,這一過程稱原代培養(yǎng)。
選購流程:
采購實驗裝備就是如此簡單。我司細(xì)胞活力狀態(tài)良好的細(xì)胞株1420多株,細(xì)胞來源可靠,背景資料清晰,代數(shù)年輕,活力好。其中絕大部分是近年來從美國ATCC和NIH分批引進(jìn)的細(xì)胞種子,少部分來自全國各地細(xì)胞研究所。
從實驗室的設(shè)計到實驗過程中的操作,都需要嚴(yán)格控制無菌。由于細(xì)胞對于生長的條件比較苛刻,所以無論從實驗室的設(shè)計,還是使用的物品及細(xì)胞培養(yǎng)的操作過程,都要注意保持無菌環(huán)境。
我司特性經(jīng)測試:具有良好的增殖和分化潛能,可分化為成骨細(xì)胞、脂肪細(xì)胞、軟骨細(xì)胞等。流式檢測結(jié)果顯示,CD29、CD44、CD73、CD105、CD166陽性,CD11a、CD34、CD45陰性。細(xì)胞培養(yǎng)基其組成成分主要有:水、氨基酸、維生素、碳水化合物、無機(jī)離子及其他一些入核酸降解物、激素等。
我司其他相關(guān)批發(fā)產(chǎn)品展示:
Cs-(025)-003162 HASMC細(xì)胞株/細(xì)胞,人大動脈平滑肌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003163 HCPEpiC細(xì)胞株/細(xì)胞,人脈絡(luò)叢上皮細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003164 VE細(xì)胞株/細(xì)胞,人血管內(nèi)皮細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003165 EC-304細(xì)胞株/細(xì)胞,人血管內(nèi)皮細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003166 HUVEC(原代)細(xì)胞株/細(xì)胞,人臍靜脈血管內(nèi)皮細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003167 HUVE-12/CRL2480細(xì)胞株/細(xì)胞,人臍靜脈內(nèi)皮細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003168 ECV-304細(xì)胞株/細(xì)胞,人臍靜脈內(nèi)皮細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003169 ECV-304細(xì)胞株/細(xì)胞,人臍靜脈內(nèi)皮(具有T24細(xì)胞株/細(xì)胞特性) ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003170 Ealy926細(xì)胞株/細(xì)胞,人腸系膜下腔動脈血管內(nèi)皮細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003171 HEB細(xì)胞株/細(xì)胞,人腦膠質(zhì)細(xì)胞株/細(xì)胞株(正常) ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003172 SF126細(xì)胞株/細(xì)胞,人腦瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003173 SF763細(xì)胞株/細(xì)胞,人腦瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003174 SF767細(xì)胞株/細(xì)胞,人腦瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003175 SF17細(xì)胞株/細(xì)胞,人腦瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003176 TG-905細(xì)胞株/細(xì)胞,人腦膠質(zhì)母細(xì)胞株/細(xì)胞瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003177 TJ905細(xì)胞株/細(xì)胞,人膠質(zhì)瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003178 IMR-32細(xì)胞株/細(xì)胞,人神經(jīng)母細(xì)胞株/細(xì)胞瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003179 U251細(xì)胞株/細(xì)胞,人神經(jīng)膠質(zhì)瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003180 CRT細(xì)胞株/細(xì)胞,神經(jīng)膠質(zhì)瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003181 A172細(xì)胞株/細(xì)胞,人膠質(zhì)母細(xì)胞株/細(xì)胞瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003182 SHG-44細(xì)胞株/細(xì)胞,神經(jīng)膠質(zhì)瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003183 SF-295細(xì)胞株/細(xì)胞,人XG惡性膠質(zhì)瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003184 U87MG細(xì)胞株/細(xì)胞,人惡性膠質(zhì)母細(xì)胞株/細(xì)胞瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003185 SK-N-MC細(xì)胞株/細(xì)胞,人神經(jīng)上皮瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003186 SK-N-SH細(xì)胞株/細(xì)胞,人神經(jīng)上皮瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003187 SH-SY5Y細(xì)胞株/細(xì)胞,人神經(jīng)上皮瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003188 H4細(xì)胞株/細(xì)胞,人腦神經(jīng)膠質(zhì)瘤細(xì)胞株/細(xì)胞(H4) ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003189 U373細(xì)胞株/細(xì)胞,人腦膠質(zhì)瘤細(xì)胞株/細(xì)胞系 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003190 SW038-C2細(xì)胞株/細(xì)胞,人腦膠質(zhì)母細(xì)胞株/細(xì)胞瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003191 SW038-C2-tk細(xì)胞株/細(xì)胞,轉(zhuǎn)染了tk基因的SW038-C2細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003192 BT-325細(xì)胞株/細(xì)胞,人神經(jīng)膠質(zhì)瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003193 BE(2)C細(xì)胞株/細(xì)胞,人神經(jīng)母細(xì)胞株/細(xì)胞瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003194 BE(2)-M17細(xì)胞株/細(xì)胞,人神經(jīng)母細(xì)胞株/細(xì)胞瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003195 HOPC-os細(xì)胞株/細(xì)胞,人少突膠質(zhì)前體細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003196 Hp2/o細(xì)胞株/細(xì)胞,人跟骨髓瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003197 U-2 OS細(xì)胞株/細(xì)胞,人骨肉瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003198 SP20細(xì)胞株/細(xì)胞,人骨肉瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003199 SW1353細(xì)胞株/細(xì)胞,人骨肉瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003200 Saos-2細(xì)胞株/細(xì)胞,人成骨肉瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003201 HOS細(xì)胞株/細(xì)胞,人骨肉瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003202 U266細(xì)胞株/細(xì)胞,人骨髓瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003203 3T3D細(xì)胞株/細(xì)胞,人骨髓瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003204 MG-63細(xì)胞株/細(xì)胞,人成骨肉瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003205 RPMI-8226細(xì)胞株/細(xì)胞,人多發(fā)性骨髓瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003206 A-204細(xì)胞株/細(xì)胞,人橫紋肌肉瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003207 FBM細(xì)胞株/細(xì)胞,胎兒骨髓間充質(zhì)細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003208 HME1細(xì)胞株/細(xì)胞,人黑色素瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003209 HME4細(xì)胞株/細(xì)胞,人黑色素瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003210 HME6細(xì)胞株/細(xì)胞,人黑色素瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(025)-003211 HME7細(xì)胞株/細(xì)胞,人黑色素瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
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