更新時間:2016-06-02
我司所提供的實驗NCI-H1703:人肺鱗癌細(xì)胞,不能用于人體實驗和臨床診斷、治療。我司細(xì)胞研究中心承諾,盡Z大努力對實驗細(xì)胞資源相關(guān)信息進(jìn)行及時更新,同時也保證純度在90%以上,咨詢購買。
免費咨詢:021-54720761
NCI-H1703:人肺鱗癌細(xì)胞培養(yǎng)方法:
收到細(xì)胞后,在倒置顯微鏡下觀察整個細(xì)胞生長情況,如果細(xì)胞未長滿,用75%酒精噴灑整個瓶消毒后放到超菌臺內(nèi),嚴(yán)格無菌操作,打開細(xì)胞培養(yǎng)瓶,留10ml培養(yǎng)液繼續(xù)培養(yǎng)。
NCI-H1703:人肺鱗癌細(xì)胞初代培養(yǎng)原理:
將動物機(jī)體的各種組織從機(jī)體中取出,經(jīng)各種酶(常用胰蛋白酶)、螯合劑(常用EDTA)或機(jī)械方法處理,分散成單細(xì)胞,置合適的培養(yǎng)基中培養(yǎng),使細(xì)胞得以生存、生長和繁殖,這一過程稱原代培養(yǎng)。
選購流程:
采購實驗裝備就是如此簡單。我司細(xì)胞活力狀態(tài)良好的細(xì)胞株1420多株,細(xì)胞來源可靠,背景資料清晰,代數(shù)年輕,活力好。其中絕大部分是近年來從美國ATCC和NIH分批引進(jìn)的細(xì)胞種子,少部分來自全國各地細(xì)胞研究所。
從實驗室的設(shè)計到實驗過程中的操作,都需要嚴(yán)格控制無菌。由于細(xì)胞對于生長的條件比較苛刻,所以無論從實驗室的設(shè)計,還是使用的物品及細(xì)胞培養(yǎng)的操作過程,都要注意保持無菌環(huán)境。
我司特性經(jīng)測試:具有良好的增殖和分化潛能,可分化為成骨細(xì)胞、脂肪細(xì)胞、軟骨細(xì)胞等。流式檢測結(jié)果顯示,CD29、CD44、CD73、CD105、CD166陽性,CD11a、CD34、CD45陰性。細(xì)胞培養(yǎng)基其組成成分主要有:水、氨基酸、維生素、碳水化合物、無機(jī)離子及其他一些入核酸降解物、激素等。
我司其他相關(guān)批發(fā)產(chǎn)品展示:
Cs-(024)-000771 HEp-2細(xì)胞株/細(xì)胞,人喉表皮樣癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000772 Hep3B2.1-7人肝癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000773 Hep3B2.1-7細(xì)胞株/細(xì)胞,人肝癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000774 Hep-3B人肝癌細(xì)胞株/細(xì)胞系 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000775 Hep3B細(xì)胞株/細(xì)胞,肝癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000776 Hep-3B細(xì)胞株/細(xì)胞,人肝癌細(xì)胞株/細(xì)胞系 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000777 Hepa 1-6(肝癌細(xì)胞株/細(xì)胞) ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000778 Hepa 1-6細(xì)胞株/細(xì)胞,肝癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000779 Hepa 1-6細(xì)胞株/細(xì)胞,小鼠肝癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000780 HEPA1-6細(xì)胞株/細(xì)胞,小鼠肝癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000781 Hepa1-6小鼠肝癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000782 HepG2.2.1.5細(xì)胞株/細(xì)胞,人肝癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000783 HepG2.2.15細(xì)胞株/細(xì)胞,表達(dá)HBV病毒的人肝細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000784 HepG2/2-15細(xì)胞株/細(xì)胞,人肝癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000785 HepG2人肝癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000786 HepG2細(xì)胞株/細(xì)胞,肝細(xì)胞株/細(xì)胞癌 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000787 HepG-2細(xì)胞株/細(xì)胞,人肝癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000788 HepII細(xì)胞株/細(xì)胞,猴腎細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000789 HFF細(xì)胞株/細(xì)胞,小兒細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000790 HFL-I(MEMα)(胚肺成纖維細(xì)胞株/細(xì)胞) ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000791 HFL-I細(xì)胞株/細(xì)胞,胚肺成纖維細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000792 HFL-I細(xì)胞株/細(xì)胞,人胚肺成纖維細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000793 hFOB 1.19(SV40 轉(zhuǎn)染成骨細(xì)胞株/細(xì)胞) ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000794 hFOB 1.19(人SV40轉(zhuǎn)染成骨細(xì)胞株/細(xì)胞) ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000795 hFOB 1.19細(xì)胞株/細(xì)胞,人SV40轉(zhuǎn)染成骨細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000796 HFSF細(xì)胞株/細(xì)胞,人胚胎眼鞏膜成纖維細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000797 HFTF細(xì)胞株/細(xì)胞,人胚胎眼Tenon's囊成纖維細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000798 HGC-27(胃癌細(xì)胞株/細(xì)胞(未分化)) ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000799 HGC-27人胃癌細(xì)胞株/細(xì)胞(未分化) ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000800 HGC-27細(xì)胞株/細(xì)胞,人未分化胃癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000801 HHCC細(xì)胞株/細(xì)胞,人肝癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000802 HIBEpiC細(xì)胞株/細(xì)胞,人肝內(nèi)膽管上皮細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000803 HIC細(xì)胞株/細(xì)胞,人小腸癌細(xì)胞株/細(xì)胞系 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000804 HIT-T15(倉鼠beta胰島細(xì)胞株/細(xì)胞) ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000805 HIT-T15細(xì)胞株/細(xì)胞,倉鼠beta胰島細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000806 HK-2C細(xì)胞株/細(xì)胞,人胚腎上皮細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000807 HK-2腎小管上皮細(xì)胞株/細(xì)胞株HK-2 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000808 HK-2細(xì)胞株/細(xì)胞,人腎小管近段上皮細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000809 HKC細(xì)胞株/細(xì)胞,人胚腎上皮細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000810 HL-60(白血病原髓細(xì)胞株/細(xì)胞) ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000811 HL-60(急性粒細(xì)胞株/細(xì)胞白血病細(xì)胞株/細(xì)胞) ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000812 HL-60/Ad細(xì)胞株/細(xì)胞,人白血病阿霉素耐藥株 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000813 HL-60人原髓細(xì)胞株/細(xì)胞白血病細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000814 HL-60細(xì)胞株/細(xì)胞,白血病原髓細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000815 HL60細(xì)胞株/細(xì)胞,人原髓細(xì)胞株/細(xì)胞白血病細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000816 HL-60細(xì)胞株/細(xì)胞,早幼粒急性白血病細(xì)胞株/細(xì)胞系 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000817 HL-7702(肝細(xì)胞株/細(xì)胞) ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000818 HL-7702人肝正常細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000819 HL-7702細(xì)胞株/細(xì)胞,肝細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000820 HL-7702細(xì)胞株/細(xì)胞,人肝細(xì)胞株/細(xì)胞正常 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000821 HLAmP細(xì)胞株/細(xì)胞,人肺巨細(xì)胞株/細(xì)胞癌細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000822 HLF-02細(xì)胞株/細(xì)胞,人胚肺二倍體細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000823 HLF細(xì)胞株/細(xì)胞,胚肺細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000824 HL細(xì)胞株/細(xì)胞,人二倍體細(xì)胞株/細(xì)胞系 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000825 HMC細(xì)胞株/細(xì)胞,人腎小球系膜細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000826 HME1細(xì)胞株/細(xì)胞,人黑色素瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000827 HME2細(xì)胞株/細(xì)胞,人黑色素瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000828 HME4細(xì)胞株/細(xì)胞,人黑色素瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000829 HME6細(xì)胞株/細(xì)胞,人黑色素瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000830 HME7細(xì)胞株/細(xì)胞,人黑色素瘤細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000831 HMy2.CIR(人B 淋巴母細(xì)胞株/細(xì)胞) ,規(guī)格: >5×100000cells/瓶
Cs-(024)-000832 HMy2.CIR人B淋巴母細(xì)胞株/細(xì)胞 ,規(guī)格: >5×100000cells/瓶
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